TVP Biomass Fermentation

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Texture

Cost & scalability

First results available

TVP biomass fermentation

Growing fungus on a TVP scaffold, aiming for mycoprotein-class functionality at closer to TVP cost.

Illustration of yellow fungal mycelium branching through a porous blue TVP scaffold, with close-ups of the hyphae and a mushroom

Stage

Proof of concept complete · stage 2 starting

Timeline

Stage 1 done in 2026 · stage 2 from Q4 2026

Execution

Stage 1 with Solid Fermentation Innovation

Open output

POC report · publication pending

The gap

Great texture, high cost

Fungal biomass brings a fibrous, layered texture that plant proteins struggle to match, but it is expensive to grow.

Submerged fermentation is controllable and scalable but gives the fungus nothing to grow into. Solid-state fermentation lets it colonize a structure, closer to how it grows in nature, but is harder to control and scale. TVP is the opposite: abundant, low-cost and food-grade, but limited by beany off-notes and texture.

$4–6 /kg

Modeled production cost of biomass-fermentation protein (GFI, 2025)

$2.70–3.66 /kg

Published wholesale prices for dry TVP (Green Circle Capital, 2024; ICI Foods list price, September 2026)

How it works

Give the fungus a scaffold to grow into

An intact TVP particle goes into the submerged fermenter, so the mycelium colonizes a structured, food-grade substrate while the process stays controllable.

Graphical abstract: conventional submerged fermentation, where mycelium grows without mechanical support, beside TVP-enhanced submerged fermentation, where a porous food-grade TVP scaffold gives the mycelium physical support and nutrient flow
Left: conventional submerged fermentation, where mycelium grows without mechanical support. Right: the TVP-enhanced process, where a porous, food-grade TVP scaffold provides physical cues and nutrient flow.

Progress so far

Where the project stands

The project runs in stages. Each stage tests what the previous one left open, so every finding is reported with the stage that produced it.

  Done

Proof of concept

Q1–Sep 2026

  Now

Controlled reactor runs

From Q4 2026

Next

Larger batches for evaluation

Scope set by stage 2

Stage 1 · Proof of concept · completed September 2026

Does the fungus grow into a TVP scaffold?

We tested four ways of combining solid-state and submerged fermentation on intact TVP particles. Every run had a twin: TVP taken through exactly the same steps without fungus, so any difference could be traced to the fungus rather than to the process.

Fermented TVP holds more water

Bar chart of water-holding capacity in grams of water per gram of dry material. TVP processed the same way without fungus held 3.7. Fermented routes: SSF-first 5.3, One-Step 48 h 5.6, One-Step 72 h 6.3, Two-Step 6.0.
Water held per gram of dry material, averaged over two TVP particle sizes (three samples each). Bars show the mean; the line marks one standard error.

Only one route ended heavier than TVP on its own

Bar chart of the change in dry mass per run compared with TVP processed without fungus: SSF-first +0.38 g, One-Step 48 h minus 0.02 g, One-Step 72 h minus 0.31 g, Two-Step minus 0.26 g.
Change in dry mass per run against TVP processed without fungus, from about 2 g of TVP per run. A gain fits new fungal growth but does not prove it.

The two measures point in opposite directions: the route that kept the most mass held the least water. That is not what fungal growth alone would predict, and it is one of the questions this stage leaves open.

What it established

A working end-to-end process. Fungus grown on intact TVP in a submerged vessel, then dried into an edible patty. We found no published report of this approach.

Higher water-holding. Every fermented route held more water than TVP processed the same way without fungus.

What it couldn’t show yet

How much of the mass is new fungus. Nothing measured yet separates new mycelium from scaffold that simply survived. A fungal-specific assay would decide it.

Whether fermentation changes the taste. Five tasters tended to rate SSF-first higher on mushroom aroma and umami, but a panel this small can’t confirm a difference. No fermented sample differed from plain TVP on soy off-notes either, so that question is open, not answered.

Which route is best. SSF-first leads on mass, but it also received extra fresh medium, so its advantage can’t yet be attributed to the route itself.

Stage 2 · Now · agreement in progress

Can the process be controlled and repeated?

Stage 2 takes the process into small, controlled bioreactors with an experienced fermentation partner. It runs in three steps:

  • Screen fungal strains on TVP, to pick the most promising combinations.
  • Set the reactor’s operating conditions before any fungus goes in.
  • Run the best condition more than once, to test whether the result can be reproduced.

As in stage 1, every comparison includes TVP processed without fungus, so changes can be traced to the fungus.

Stage 3 · Next

Is the material ready to be evaluated as food?

If stage 2 finds a condition that holds up and repeats, the next step is producing larger batches for evaluation, including a tasting panel once the material is cleared for food use.

Who’s involved

How the work is split

Labs by GFI Israel

Project lead

Project design, scientific direction, data analysis and the open-access report.

Technology partner · stage 1

Solid Fermentation Innovation (SFI)

Executed the proof-of-concept fermentations.

Fermentation partner · stage 2

Agreement in progress

An experienced fermentation partner will run the reactor work.

Talk to the project lead

Ariel Szklanny, PhD

Project Manager · labs@gfi.org

Last updated: October 2026

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Labs by GFI Israel | A GFI Israel initiative | Complementary to GFI’s global alternative protein mission

[LinkedIn] · Contact: labs@gfi.org